Interferon priming enables cells to partially overturn the SARS coronavirus-induced block in innate immune activation - Forschungsportal der Justus-Liebig-Universität Gießen:

Journalartikel

Interferon priming enables cells to partially overturn the SARS coronavirus-induced block in innate immune activation

Autorenliste: Kuri, T; Zhang, XN; Habjan, M; Martínez-Sobrido, L; García-Sastre, A; Yuan, ZH; Weber, F

Jahr der Veröffentlichung: 2009

Seiten: 2686-2694

Zeitschrift: Journal of General Virology

Bandnummer: 90

ISSN: 0022-1317

eISSN: 1465-2099

DOI Link: https://doi.org/10.1099/vir.0.013599-0

Verlag: Microbiology Society

Abstract:
SARS coronavirus (SARS-CoV) is known to efficiently suppress the induction of antiviral type I interferons (IFN-alpha/beta) in non-lymphatic cells through inhibition of the transcription factor IRF-3. Plasmacytoid dendritic cells, in contrast, respond to infection with production of high levels of IFNs. Here, we show that pretreatment of non-lymphatic cells with small amounts of IFN-alpha (IFN priming) partially overturns the block in IFN induction imposed by SARS-CoV. IFN priming combined with SARS-CoV infection substantially induced genes for IFN induction, IFN signalling, antiviral effector proteins, ubiquitination and ISGylation, antigen presentation and other cytokines and chemokines, whereas each individual treatment had no major effect. Curiously, however, despite this typical IFN response, neither IRF-3 nor IRF-7 was transported to the nucleus as a sign of activation. Taken together, our results suggest that (i) IFN, as it is produced by plasmacytoid dendritic cells, could enable tissue cells to launch a host response to SARS-CoV, (ii) IRF-3 and IRF-7 may be active at subdetectable levels, and (iii) SARS-CoV does not activate IRF-7.

Autoren/Herausgeber

- Uni.-Prof. Dr. Friedemann Johannes Weber

Zitierstile

Harvard-Zitierstil: Kuri, T., Zhang, X., Habjan, M., Martínez-Sobrido, L., García-Sastre, A., Yuan, Z., et al. (2009) Interferon priming enables cells to partially overturn the SARS coronavirus-induced block in innate immune activation, Journal of General Virology, 90, pp. 2686-2694. https://doi.org/10.1099/vir.0.013599-0

APA-Zitierstil: Kuri, T., Zhang, X., Habjan, M., Martínez-Sobrido, L., García-Sastre, A., Yuan, Z., & Weber, F. (2009). Interferon priming enables cells to partially overturn the SARS coronavirus-induced block in innate immune activation. Journal of General Virology. 90, 2686-2694. https://doi.org/10.1099/vir.0.013599-0