Journal article

Authors list: Vogt, C; Preuss, E; Mayer, D; Weber, F; Schwemmle, M; Kochs, G

Publication year: 2008

Pages: 11446-11453

Journal: Journal of Virology

Volume number: 82

Issue number: 22

ISSN: 0022-538X

eISSN: 1098-5514

DOI Link: https://doi.org/10.1128/JVI.01284-08

Publisher: American Society for Microbiology

Abstract: 
The ML protein of Thogoto virus, a tick-transmitted orthomyxovirus, is a splice variant of the viral matrix protein and antagonizes the induction of antiviral type I interferon (IFN). Here we identified the general RNA polymerase II transcription factor IIB (TFIIB) as an ML-interacting protein. Overexpression of TFIIB neutralized the inhibitory effect of ML on IRF3-mediated promoter activation. Moreover, a recombinant virus expressing a mutant ML protein unable to bind TFIIB was severely impaired in its ability to suppress IFN induction. We concluded that TFIIB binding is required for the IFN antagonist effect exerted by ML. We further demonstrate that the ML-TFIIB interaction has surprisingly little impact on gene expression in general, while a strong negative effect is observed for IRF3- and NF-kappa B-regulated promoters.

Harvard Citation style: Vogt, C., Preuss, E., Mayer, D., Weber, F., Schwemmle, M. and Kochs, G. (2008) The Interferon Antagonist ML Protein of Thogoto Virus Targets General Transcription Factor IIB, Journal of Virology, 82(22), pp. 11446-11453. https://doi.org/10.1128/JVI.01284-08

APA Citation style: Vogt, C., Preuss, E., Mayer, D., Weber, F., Schwemmle, M., & Kochs, G. (2008). The Interferon Antagonist ML Protein of Thogoto Virus Targets General Transcription Factor IIB. Journal of Virology. 82(22), 11446-11453. https://doi.org/10.1128/JVI.01284-08