Journalartikel

Autorenliste: Kuri, T; Habjan, M; Penski, N; Weber, F

Jahr der Veröffentlichung: 2010

Zeitschrift: Virology Journal

Bandnummer: 7

ISSN: 1743-422X

DOI Link: https://doi.org/10.1186/1743-422X-7-50

Verlag: BioMed Central

Abstract: 
Background

Studies of the host response to infection often require quantitative measurement of the antiviral type I interferons (IFN-α/β) in biological samples. The amount of IFN is either determined via its ability to suppress a sensitive indicator virus, by an IFN-responding reporter cell line, or by ELISA. These assays however are either time-consuming and lack convenient readouts, or they are rather insensitive and restricted to IFN from a particular host species.

Results

An IFN-sensitive, Renilla luciferase-expressing Rift Valley fever virus (RVFV-Ren) was generated using reverse genetics. Human, murine and avian cells were tested for their susceptibility to RVFV-Ren after treatment with species-specific IFNs. RVFV-Ren was able to infect cells of all three species, and IFN-mediated inhibition of viral reporter activity occurred in a dose-dependent manner. The sensitivity limit was found to be 1 U/ml IFN, and comparison with a standard curve allowed to determine the activity of an unknown sample.

Conclusions

RVFV-Ren replicates in cells of several species and is highly sensitive to pre-treatment with IFN. These properties allowed the development of a rapid, sensitive, and species-independent antiviral assay with a convenient luciferase-based readout.

Harvard-Zitierstil: Kuri, T., Habjan, M., Penski, N. and Weber, F. (2010) Species-independent bioassay for sensitive quantification of antiviral type I interferons, Virology Journal, 7, Article 50. https://doi.org/10.1186/1743-422X-7-50

APA-Zitierstil: Kuri, T., Habjan, M., Penski, N., & Weber, F. (2010). Species-independent bioassay for sensitive quantification of antiviral type I interferons. Virology Journal. 7, Article 50. https://doi.org/10.1186/1743-422X-7-50