Journal article

Thogoto virus ML protein is a potent inhibitor of the interferon regulatory factor-7 transcription factor


Authors listBuettner, N; Vogt, C; Martínez-Sobrido, L; Weber, F; Waibler, Z; Kochs, G

Publication year2010

Pages220-227

JournalJournal of General Virology

Volume number91

Issue number1

ISSN0022-1317

eISSN1465-2099

DOI Linkhttps://doi.org/10.1099/vir.0.015172-0

PublisherMicrobiology Society


Abstract

The tick-transmitted orthomyxovirus Thogoto virus (THOV) encodes the ML protein acting as a viral suppressor of the host interferon (IFN) system. Here, we describe that type I IFN is strongly induced in primary mouse embryo fibroblasts as well as plasmacytoid dendritic cells upon infection with a THOV mutant lacking the ML gene. However, wild-type THOV encoding ML suppresses induction of IFN by preventing the activation of members of the IFN regulatory factor (IRF) family. We found that reporter gene expression dependent on IRF3 and IRF7 was strongly inhibited by ML. Further experiments revealed that ML interacts with IRF7 and prevents dimerization of the transcription factor and its association with the coactivator TRAF6. Interestingly, another IRF7 activation step, nuclear translocation, is not affected by ML. Our data elucidate ML protein as a virulence factor with an IRF-specific IFN-antagonistic spectrum.




Citation Styles

Harvard Citation styleBuettner, N., Vogt, C., Martínez-Sobrido, L., Weber, F., Waibler, Z. and Kochs, G. (2010) Thogoto virus ML protein is a potent inhibitor of the interferon regulatory factor-7 transcription factor, Journal of General Virology, 91(1), pp. 220-227. https://doi.org/10.1099/vir.0.015172-0

APA Citation styleBuettner, N., Vogt, C., Martínez-Sobrido, L., Weber, F., Waibler, Z., & Kochs, G. (2010). Thogoto virus ML protein is a potent inhibitor of the interferon regulatory factor-7 transcription factor. Journal of General Virology. 91(1), 220-227. https://doi.org/10.1099/vir.0.015172-0


Last updated on 2025-21-05 at 17:06