Journal article

Authors list: Glowacka, I; Bertram, S; Herzog, P; Pfefferle, S; Steffen, I; Muench, MO; Simmons, G; Hofmann, H; Kuri, T; Weber, F; Eichler, J; Drosten, C; Pöhlmann, S

Publication year: 2010

Pages: 1198-1205

Journal: Journal of Virology

Volume number: 84

Issue number: 2

ISSN: 0022-538X

eISSN: 1098-5514

DOI Link: https://doi.org/10.1128/JVI.01248-09

Publisher: American Society for Microbiology

Abstract: 

The human coronaviruses (CoVs) severe acute respiratory syndrome (SARS)-CoV and NL63 employ angiotensin-converting enzyme 2 (ACE2) for cell entry. It was shown that recombinant SARS-CoV spike protein (SARS-S) downregulates ACE2 expression and thereby promotes lung injury. Whether NL63-S exerts a similar activity is yet unknown. We found that recombinant SARS-S bound to ACE2 and induced ACE2 shedding with higher efficiency than NL63-S. Shedding most likely accounted for the previously observed ACE2 downregulation but was dispensable for viral replication. Finally, SARS-CoV but not NL63 replicated efficiently in ACE2-positive Vero cells and reduced ACE2 expression, indicating robust receptor interference in the context of SARS-CoV but not NL63 infection.

Harvard Citation style: Glowacka, I., Bertram, S., Herzog, P., Pfefferle, S., Steffen, I., Muench, M., et al. (2010) Differential Downregulation of ACE2 by the Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus and Human Coronavirus NL63, Journal of Virology, 84(2), pp. 1198-1205. https://doi.org/10.1128/JVI.01248-09

APA Citation style: Glowacka, I., Bertram, S., Herzog, P., Pfefferle, S., Steffen, I., Muench, M., Simmons, G., Hofmann, H., Kuri, T., Weber, F., Eichler, J., Drosten, C., & Pöhlmann, S. (2010). Differential Downregulation of ACE2 by the Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus and Human Coronavirus NL63. Journal of Virology. 84(2), 1198-1205. https://doi.org/10.1128/JVI.01248-09