Completion of Hepatitis C Virus Replication Cycle in Heterokaryons Excludes Dominant Restrictions in Human Non-liver and Mouse Liver Cell Lines - Research portal of Justus Liebig University Giessen:

Journal article

Completion of Hepatitis C Virus Replication Cycle in Heterokaryons Excludes Dominant Restrictions in Human Non-liver and Mouse Liver Cell Lines

Authors list: Frentzen, A; Hüging, K; Bitzegeio, J; Friesland, M; Haid, S; Gentzsch, J; Hoffmann, M; Lindemann, D; Zimmer, G; Zielecki, F; Weber, F; Steinmann, E; Pietschmann, T

Publication year: 2011

Journal: PLoS Pathogens

Volume number: 7

Issue number: 4

ISSN: 1553-7366

eISSN: 1553-7374

DOI Link: https://doi.org/10.1371/journal.ppat.1002029

Publisher: Public Library of Science

Abstract:

Hepatitis C virus (HCV) is hepatotropic and only infects humans and chimpanzees. Consequently, an immunocompetent small animal model is lacking. The restricted tropism of HCV likely reflects specific host factor requirements. We investigated if dominant restriction factors expressed in non-liver or non-human cell lines inhibit HCV propagation thus rendering these cells non-permissive. To this end we explored if HCV completes its replication cycle in heterokaryons between human liver cell lines and non-permissive cell lines from human non-liver or mouse liver origin. Despite functional viral pattern recognition pathways and responsiveness to interferon, virus production was observed in all fused cells and was only ablated when cells were treated with exogenous interferon. These results exclude that constitutive or virus-induced expression of dominant restriction factors prevents propagation of HCV in these cell types, which has important implications for HCV tissue and species tropism. In turn, these data strongly advocate transgenic approaches of crucial human HCV cofactors to establish an immunocompetent small animal model.

Authors/Editors

- Uni.-Prof. Dr. Friedemann Johannes Weber

Citation Styles

Harvard Citation style: Frentzen, A., Hüging, K., Bitzegeio, J., Friesland, M., Haid, S., Gentzsch, J., et al. (2011) Completion of Hepatitis C Virus Replication Cycle in Heterokaryons Excludes Dominant Restrictions in Human Non-liver and Mouse Liver Cell Lines, PLoS Pathogens, 7(4), Article e1002029. https://doi.org/10.1371/journal.ppat.1002029

APA Citation style: Frentzen, A., Hüging, K., Bitzegeio, J., Friesland, M., Haid, S., Gentzsch, J., Hoffmann, M., Lindemann, D., Zimmer, G., Zielecki, F., Weber, F., Steinmann, E., & Pietschmann, T. (2011). Completion of Hepatitis C Virus Replication Cycle in Heterokaryons Excludes Dominant Restrictions in Human Non-liver and Mouse Liver Cell Lines. PLoS Pathogens. 7(4), Article e1002029. https://doi.org/10.1371/journal.ppat.1002029