A universal SARS-CoV DNA vaccine inducing highly cross-reactive neutralizing antibodies and T cells - Research portal of Justus Liebig University Giessen:

Journal article

A universal SARS-CoV DNA vaccine inducing highly cross-reactive neutralizing antibodies and T cells

Authors list: Appelberg, S; Ahlén, G; Yan, J; Nikouyan, N; Weber, S; Larsson, O; Höglund, U; Aleman, S; Weber, F; Perlhamre, E; Apro, J; Gidlund, EK; Tuvesson, O; Salati, S; Cadossi, M; Tegel, H; Hober, S; Frelin, L; Mirazimi, A; Sällberg, M

Publication year: 2022

Journal: EMBO Molecular Medicine

Volume number: 14

Issue number: 10

ISSN: 1757-4676

eISSN: 1757-4684

DOI Link: https://doi.org/10.15252/emmm.202215821

Publisher: Springer

Abstract:

New variants in the SARS-CoV-2 pandemic are more contagious (Alpha/Delta), evade neutralizing antibodies (Beta), or both (Omicron). This poses a challenge in vaccine development according to WHO. We designed a more universal SARS-CoV-2 DNA vaccine containing receptor-binding domain loops from the huCoV-19/WH01, the Alpha, and the Beta variants, combined with the membrane and nucleoproteins. The vaccine induced spike antibodies crossreactive between huCoV-19/WH01, Beta, and Delta spike proteins that neutralized huCoV-19/WH01, Beta, Delta, and Omicron virus in vitro. The vaccine primed nucleoprotein-specific T cells, unlike spike-specific T cells, recognized Bat-CoV sequences. The vaccine protected mice carrying the human ACE2 receptor against lethal infection with the SARS-CoV-2 Beta variant. Interestingly, priming of cross-reactive nucleoprotein-specific T cells alone was 60% protective, verifying observations from humans that T cells protect against lethal disease. This SARS-CoV vaccine induces a uniquely broad and functional immunity that adds to currently used vaccines.

Authors/Editors

- Uni.-Prof. Dr. Friedemann Johannes Weber

Citation Styles

Harvard Citation style: Appelberg, S., Ahlén, G., Yan, J., Nikouyan, N., Weber, S., Larsson, O., et al. (2022) A universal SARS-CoV DNA vaccine inducing highly cross-reactive neutralizing antibodies and T cells, EMBO Molecular Medicine, 14(10), Article e15821. https://doi.org/10.15252/emmm.202215821

APA Citation style: Appelberg, S., Ahlén, G., Yan, J., Nikouyan, N., Weber, S., Larsson, O., Höglund, U., Aleman, S., Weber, F., Perlhamre, E., Apro, J., Gidlund, E., Tuvesson, O., Salati, S., Cadossi, M., Tegel, H., Hober, S., Frelin, L., Mirazimi, A., ...Sällberg, M. (2022). A universal SARS-CoV DNA vaccine inducing highly cross-reactive neutralizing antibodies and T cells. EMBO Molecular Medicine. 14(10), Article e15821. https://doi.org/10.15252/emmm.202215821