Journal article

Authors list: Breuer, J; Barth, P; Noe, Y; Shalamova, L; Goesmann, A; Weber, F; Rossbach, O

Publication year: 2022

Pages: 623-635

Journal: Molecular Therapy: Nucleic Acids

Volume number: 28

ISSN: 2162-2531

Open access status: Gold

DOI Link: https://doi.org/10.1016/j.omtn.2022.04.017

Publisher: Cell Press

Abstract: 

Natural circular RNAs have been found to sequester microRNAs and suppress their function. We have used this principle as a molecular tool and produced artificial circular RNA sponges in a cell-free system by in vitro transcription and ligation. Formerly, we were able to inhibit hepatitis C virus propagation by applying a circular RNA decoy strategy against microRNA-122, which is essential for the viral life cycle. In another proof-of-principle study, we used circular RNAs to sequester microRNA-21, an oncogenic and pro-proliferative microRNA. This strategy slowed tumor growth in a 3D cell culture model system, as well as in xenograft mice upon systemic delivery. In the wake of the global use of an in vitro transcribed RNA in coronavirus disease 2019 tic circular RNAs trigger cellular antiviral defense mechanisms when delivered systemically. In this study, we present data on the cellular innate immune response as a consequence of liposome-based transfection of the circular RNA We find that circular RNAs produced by the presented methodology do not trigger the antiviral response and do not activate innate immune-signaling pathways.

Harvard Citation style: Breuer, J., Barth, P., Noe, Y., Shalamova, L., Goesmann, A., Weber, F., et al. (2022) What goes around comes around: Artificial circular RNAs bypass cellular antiviral responses, Molecular Therapy: Nucleic Acids, 28, pp. 623-635. https://doi.org/10.1016/j.omtn.2022.04.017

APA Citation style: Breuer, J., Barth, P., Noe, Y., Shalamova, L., Goesmann, A., Weber, F., & Rossbach, O. (2022). What goes around comes around: Artificial circular RNAs bypass cellular antiviral responses. Molecular Therapy: Nucleic Acids. 28, 623-635. https://doi.org/10.1016/j.omtn.2022.04.017