Journal article

Authors list: Mügge, Fernanda L. B.; Sim, Cheul Muu; Honermeier, Bernd; Morlock, Gertrud E.

Publication year: 2023

Journal: International Journal of Molecular Sciences

Volume number: 24

Issue number: 12

ISSN: 1661-6596

eISSN: 1422-0067

Open access status: Gold

DOI Link: https://doi.org/10.3390/ijms24129936

Publisher: MDPI

Abstract: 

Gastrodia elata (Orchidaceae) is native to mountainous areas of Asia and is a plant species used in traditional medicine for more than two thousand years. The species was reported to have many biological activities, such as neuroprotective, antioxidant, and anti-inflammatory activity. After many years of extensive exploitation from the wild, the plant was added to lists of endangered species. Since its desired cultivation is considered difficult, innovative cultivation methods that can reduce the costs of using new soil in each cycle and at the same time avoid contamination with pathogens and chemicals are urgently needed on large scale. In this work, five G. elata samples cultivated in a facility utilizing electron beam-treated soil were compared to two samples grown in the field concerning their chemical composition and bioactivity. Using hyphenated high-performance thin-layer chromatography (HPTLC) and multi-imaging (UV/Vis/FLD, also after derivatization), the chemical marker compound gastrodin was quantified in the seven G. elata rhizome/tuber samples, which showed differences in their contents between facility and field samples and between samples collected during different seasons. Parishin E was also found to be present. Combining HPTLC with on-surface (bio)assays, the antioxidant activity and inhibition of acetylcholinesterase as well as the absence of cytotoxicity against human cells were demonstrated and compared between samples.

Harvard Citation style: Mügge, F., Sim, C., Honermeier, B. and Morlock, G. (2023) Bioactivity Profiling and Quantification of Gastrodin in Gastrodia elata Cultivated in the Field versus Facility via Hyphenated High-Performance Thin-Layer Chromatography, International Journal of Molecular Sciences, 24(12), Article 9936. https://doi.org/10.3390/ijms24129936

APA Citation style: Mügge, F., Sim, C., Honermeier, B., & Morlock, G. (2023). Bioactivity Profiling and Quantification of Gastrodin in Gastrodia elata Cultivated in the Field versus Facility via Hyphenated High-Performance Thin-Layer Chromatography. International Journal of Molecular Sciences. 24(12), Article 9936. https://doi.org/10.3390/ijms24129936