Journal article

Authors list: Lick, Sonja; Wibberg, Daniel; Winkler, Annika; Blom, Jochen; Grimmler, Christina; Goesmann, Alexander; Kalinowski, Jörn; Kröckel, Lothar

Publication year: 2021

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 71

Issue number: 6

ISSN: 1466-5026

eISSN: 1466-5034

DOI Link: https://doi.org/10.1099/ijsem.0.004822

Publisher: Microbiology Society

Abstract: 

A polyphasic approach was applied to investigate the diversity of microbiota that evolved during cold storage beef ripening. Isolate V4/DAB/S4/2a(T) with a unique BOX-rep-PCR fingerprint profile revealed more than 99 % nucleotide identities upon pairwise comparisons of 16S rDNA sequences from the type strains Pseudomonas versuta DSM 101070(T), Pseudomonas saxonica DSM 108989(T), Pseudomonas deceptionensis DSM 26521(T) and Pseudomonas weihenstephanensis DSM 29166(T), placing it within the Pseudomonas fragi / lundensis branch of the genus Pseudomonas. Additional rpoB based comparison revealed P. versuta DSM 101070(T) as the nearest relative, with 98.5 % nucleotide identity. Calculation of ANIb values of the V4/DAB/S4/2a(T) draft genome identified P. versuta DSM 101070(T) with 90.1 %, P. deceptionensis DSM 26521(T) with 85.1 %, P. fragi DSM 3456(T) with 84.4%, Pseudomonas psychrophila DSM 17535T and Pseudomonas bubulae DSM 107389(T) with 84.2 % similarities each. Pairwise genome-to-genome distance calculations [digital DNA-DNA hybridization (dDDH)] resulted in values of 47.1, 35.1, 34.8, 34.2 and 34.1 %, respectively. A second isolate was detected years later in ground beef and showed ANIb values of 99.3 % and dDDH of 96.1 % relatedness to V4/DAB/S4/2a(T). The DNA G+C content was 58.6 mol% for both isolates. The predominant cellular fatty acids of V4/DAB/S4/2a(T) were C16:0, C18:1 omega 7c, C17:0 cyclo and a summed feature containing C16:1 omega 7c and/or C15: 0 iso 2 OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, the major respiratory quinone was Q9, with a small portion of Q8. The combined data on genotypic and phenotypic features support the proposal of a novel species, for which the name Pseudomonas paraversuta sp. nov. is proposed. The type strain is V4/DAB/S4/2a(T) (=DSM 111361(T)=LMG 31844(T)) and a second isolate is UBT376 (=DSM 111360=LMG 31845).

Harvard Citation style: Lick, S., Wibberg, D., Winkler, A., Blom, J., Grimmler, C., Goesmann, A., et al. (2021) Pseudomonas paraversuta sp. nov. isolated from refrigerated dry-aged beef, International Journal of Systematic and Evolutionary Microbiology, 71(6), Article 0004822. https://doi.org/10.1099/ijsem.0.004822

APA Citation style: Lick, S., Wibberg, D., Winkler, A., Blom, J., Grimmler, C., Goesmann, A., Kalinowski, J., & Kröckel, L. (2021). Pseudomonas paraversuta sp. nov. isolated from refrigerated dry-aged beef. International Journal of Systematic and Evolutionary Microbiology. 71(6), Article 0004822. https://doi.org/10.1099/ijsem.0.004822