Journal article

Authors list: Kishore, A; Fan, L; Stahl, F; Reichel, T; Krüger, K; Zeilinger, C

Publication year: 2023

Journal: Applied Sciences

Volume number: 13

Issue number: 5

eISSN: 2076-3417

Open access status: Gold

DOI Link: https://doi.org/10.3390/app13052957

Publisher: MDPI

Abstract: 

HSP90 alpha is one of the most common stress proteins in cells; hence, it is a good target for developing drugs and testing systems for cancer or physical stress levels in humans. Streptavidin conjugated quantum dots (Sav-QDs) are widely used as fluorophores for biosensing to overcome chemical labelling problems. In this work, we have attempted to develop a multifunctional and robust assay for HSP90 alpha. The detection technique was based on the masking of the fluorescence of spotted Sav-QDs on nitrocellulose chips (NC). Biotinylated ligand/antibody attaches to the spotted Sav-QD and then HSP90 alpha is attached, which causes the masking of fluorescence. The masking of fluorescence was used to detect protein-ligand interactions, the effect of inhibitors, protein-protein interactions, and the presence of protein in the biological sample. The load of detection (LoD) of the assay lies in the nano molar range, making it a sensitive assay. The results from the experiments suggest that the used approach is promising for developing a multifunctional, robust, and sensitive assay for proteins that can be used for point-of-care detection in complex biological samples.

Harvard Citation style: Kishore, A., Fan, L., Stahl, F., Reichel, T., Krüger, K. and Zeilinger, C. (2023) Fluorescence Masking Based Multifunctional Quantum Dots' Assay for HSP90α Interactions Detection, Applied Sciences, 13(5), Article 2957. https://doi.org/10.3390/app13052957

APA Citation style: Kishore, A., Fan, L., Stahl, F., Reichel, T., Krüger, K., & Zeilinger, C. (2023). Fluorescence Masking Based Multifunctional Quantum Dots' Assay for HSP90α Interactions Detection. Applied Sciences. 13(5), Article 2957. https://doi.org/10.3390/app13052957