Journal article

Authors list: Kramer, Jil-Christine; Böhringer, Nils; Mettal, Ute; Schäberle, Till F.

Publication year: 2023

Journal: STAR Protocols

Volume number: 4

Issue number: 3

ISSN: 2666-1667

Open access status: Gold

DOI Link: https://doi.org/10.1016/j.xpro.2023.102531

Publisher: Cell Press

Abstract: 

Biosynthetic gene clusters of natural products often harbor genes of unknown function, which are difficult to characterize. Here, we present a protocol for the functional analysis in vitro and in vivo of these biosynthetic genes by heterologous expression in E. coli. We describe steps for the expression of genes of interest in an established E. coli strain optimized to heterologously express natural products. We then detail the expression of a His-tagged gene to deduce the specific function of the protein. For complete details on the use and execution of this protocol, please refer to Bohringer et al.

Harvard Citation style: Kramer, J., Böhringer, N., Mettal, U. and Schäberle, T. (2023) Functional in vitro and in vivo analysis of biosynthetic genes by heterologous expression in E. coli, STAR Protocols, 4(3), Article 102531. https://doi.org/10.1016/j.xpro.2023.102531

APA Citation style: Kramer, J., Böhringer, N., Mettal, U., & Schäberle, T. (2023). Functional in vitro and in vivo analysis of biosynthetic genes by heterologous expression in E. coli. STAR Protocols. 4(3), Article 102531. https://doi.org/10.1016/j.xpro.2023.102531