Sammelbandbeitrag

Autorenliste: Kresna, I.D.M.; Wuisan, Z.G.; Schäberle, TF

Erschienen in: Antibiotics. Methods and Protocols

Herausgeberliste: Sass, Peter

Jahr der Veröffentlichung: 2023

Seiten: 55-73

ISBN: 978-1-0716-2854-6

eISBN: 978-1-0716-2855-3

DOI Link: https://doi.org/10.1007/978-1-0716-2855-3_4

Auflage: 2nd edition

Serientitel: Methods in Molecular Biology

Serienzählung: 2601

Abstract: 

Natural product discovery campaigns aim to identify compounds with the desired bioactivity, for example, metabolites with antibiotic activity. The major driver of many projects is still the finding of bioactive extracts, which will be followed up to isolate the activity-causing agent as pure compound. However, nowadays also additional strategies can be used to increase the probability of success. Metabolomic approaches indicate chemical novelty, and genomics allow identification of putative biosynthetic gene clusters (BGCs) of interest, even though the corresponding metabolite is unknown. Whatever the entry to the campaign is, at one point the scientists need to have the desired compound in hand to analyze it in detail. Hence, expression must be achieved to yield the compound of interest, either to link it to the corresponding putative BGC or to overcome the bottleneck of sparse compound supply. Therefore, homologous and heterologous expression approaches are feasible ways forward to increase production yield, shorten fermentation time, or to get BGCs expressed at all for which no suitable fermentation condition was identified.
In this chapter, expression approaches in bacteria are described to biosynthesize compounds of interest. Homologous expression, by genetic manipulation of the original Streptomyces producer strain, and heterologous expression in the microbial workhorse Escherichia coli are exemplified.

Harvard-Zitierstil: Kresna, I., Wuisan, Z. and Schäberle, T. (2023) Production of Antimicrobial Compounds by Homologous and Heterologous Expression, in Sass, P. (ed.) Antibiotics. Methods and Protocols. 2nd edition. New York: Humana Press, pp. 55-73. https://doi.org/10.1007/978-1-0716-2855-3_4

APA-Zitierstil: Kresna, I., Wuisan, Z., & Schäberle, T. (2023). Production of Antimicrobial Compounds by Homologous and Heterologous Expression. In Sass, P. (Ed.), Antibiotics. Methods and Protocols (2nd edition, pp. 55-73). Humana Press. https://doi.org/10.1007/978-1-0716-2855-3_4