Journalartikel

Autorenliste: Gerken, BM; Nicolai, A; Linke, D; Zorn, H; Berger, RG; Parlar, H

Jahr der Veröffentlichung: 2006

Seiten: 291-294

Zeitschrift: Separation and Purification Technology

Bandnummer: 49

Heftnummer: 3

ISSN: 1383-5866

DOI Link: https://doi.org/10.1016/j.seppur.2005.09.015

Verlag: Elsevier

Abstract: 
An increasing demand of lignin-degrading enzymes makes an effective enrichment of laccase C from culture broth necessary. In this work, the continuous foam fractionation of laccase C was investigated as an alternative to other common applied methods, such as salting-out, dialyses or ultratiltration, which are usually accompanied by losses of catalytic activity or low operational capacity. After optimization of different process parameters (feed position, gas flow rate and surfactant concentration) an enrichment ratio of 11.7 was achieved for laccase C along with 70.2% recovery. Feed position at the top of the foaming column increased the recovery. Increasing the gas flow rate led to a lower enrichment but higher recovery, and an increased outlet flow rate had only negative effect on the recovery. With increasing concentration of cetyl trimethyl ammonium bromide (CTAB), which was applied as a surfactant to enable foaming, the enrichment ratio decreased (from 11.7 to 1.8) and recovery increased (from 70.2 up to 80.2%). A for the enrichment ratio and recovery optimum CTAB-concentration of 0.4 mg/ml was found. The method has been proven to be effective for the enrichment of laccase C.

Harvard-Zitierstil: Gerken, B., Nicolai, A., Linke, D., Zorn, H., Berger, R. and Parlar, H. (2006) Effective enrichment and recovery of laccase C using continuous foam fractionation, Separation and Purification Technology, 49(3), pp. 291-294. https://doi.org/10.1016/j.seppur.2005.09.015

APA-Zitierstil: Gerken, B., Nicolai, A., Linke, D., Zorn, H., Berger, R., & Parlar, H. (2006). Effective enrichment and recovery of laccase C using continuous foam fractionation. Separation and Purification Technology. 49(3), 291-294. https://doi.org/10.1016/j.seppur.2005.09.015