Journalartikel

Autorenliste: Zorn, H; Neuser, F; Berger, RG

Jahr der Veröffentlichung: 2004

Seiten: 255-263

Zeitschrift: Journal of Biotechnology

Bandnummer: 107

Heftnummer: 3

ISSN: 0168-1656

DOI Link: https://doi.org/10.1016/j.jbiotec.2003.10.002

Verlag: Elsevier

Abstract: 
When submerged cultured Pseudomonas fluorescens NCIMB 11761 was fed-batch supplemented with a-pinene oxide, a rapid formation of 2,6-dimethyl-5-methylene-hept-(2Z)-enal (I) (isonovalal) was observed. Biotransformation and isomerisation of (I) to the (2E)-isomer (II) (novalal) were enhanced by Lewatit OC 1064, a macroporous polystyrene adsorbent. Accelerated isomerisation in the presence of an amino donor (glycine) at pH 7.3 pointed to a merely chemical mechanism. A maximum yield of 48 g of aldehydes 1(-1) was achieved, but quantitative analysis of the volatile fraction showed that the molar conversion of the pinene oxide substrate reached no more than 67%. To fill this gap of the mass balance, the acidic fraction was isolated. It contained several compounds which suggested a beta-oxidation-like catabolism starting from 2,6-dimethyl-5-methylene-kept-(2E)-enoic acid (III) (novalic acid). Using [H-2(7)]-2,5,6-dimethyl-kept-(2E)-enoic acid as a conversion substrate and gas chromatography coupled to atomic emission detection and mass spectrometry a degradation pathway via labelled 3,4-dimethylpentenoic and methylpropanoic acids was evidenced. This pathway may play a predominant role in isoprenoid degradation by soil bacteria.

Harvard-Zitierstil: Zorn, H., Neuser, F. and Berger, R. (2004) Degradation of alpha-pinene oxide and [H-2(7)]-2,5,6-trimethyl-hept-(2E)-enoic acid by Pseudomonas fluorescens NCIMB 11761, Journal of Biotechnology, 107(3), pp. 255-263. https://doi.org/10.1016/j.jbiotec.2003.10.002

APA-Zitierstil: Zorn, H., Neuser, F., & Berger, R. (2004). Degradation of alpha-pinene oxide and [H-2(7)]-2,5,6-trimethyl-hept-(2E)-enoic acid by Pseudomonas fluorescens NCIMB 11761. Journal of Biotechnology. 107(3), 255-263. https://doi.org/10.1016/j.jbiotec.2003.10.002