Journalartikel

Autorenliste: Buettner, N; Vogt, C; Martínez-Sobrido, L; Weber, F; Waibler, Z; Kochs, G

Jahr der Veröffentlichung: 2010

Seiten: 220-227

Zeitschrift: Journal of General Virology

Bandnummer: 91

Heftnummer: 1

ISSN: 0022-1317

eISSN: 1465-2099

DOI Link: https://doi.org/10.1099/vir.0.015172-0

Verlag: Microbiology Society

Abstract: 

The tick-transmitted orthomyxovirus Thogoto virus (THOV) encodes the ML protein acting as a viral suppressor of the host interferon (IFN) system. Here, we describe that type I IFN is strongly induced in primary mouse embryo fibroblasts as well as plasmacytoid dendritic cells upon infection with a THOV mutant lacking the ML gene. However, wild-type THOV encoding ML suppresses induction of IFN by preventing the activation of members of the IFN regulatory factor (IRF) family. We found that reporter gene expression dependent on IRF3 and IRF7 was strongly inhibited by ML. Further experiments revealed that ML interacts with IRF7 and prevents dimerization of the transcription factor and its association with the coactivator TRAF6. Interestingly, another IRF7 activation step, nuclear translocation, is not affected by ML. Our data elucidate ML protein as a virulence factor with an IRF-specific IFN-antagonistic spectrum.

Harvard-Zitierstil: Buettner, N., Vogt, C., Martínez-Sobrido, L., Weber, F., Waibler, Z. and Kochs, G. (2010) Thogoto virus ML protein is a potent inhibitor of the interferon regulatory factor-7 transcription factor, Journal of General Virology, 91(1), pp. 220-227. https://doi.org/10.1099/vir.0.015172-0

APA-Zitierstil: Buettner, N., Vogt, C., Martínez-Sobrido, L., Weber, F., Waibler, Z., & Kochs, G. (2010). Thogoto virus ML protein is a potent inhibitor of the interferon regulatory factor-7 transcription factor. Journal of General Virology. 91(1), 220-227. https://doi.org/10.1099/vir.0.015172-0